Table 1. Primer sequences.

Gene name	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Annealing temperature
LEF1	AAGCCCAGCACTTGAATTGT	ATGACAGTTTTGGGCAAAGG	62 °C
ZIC2	CTAATCTCCATGCCCACGTT	GAACGCAATCCGGAGTTTTA	59 °C
MSX1	GAACGCAATCCGGAGTTTTA	CAGGAGACATGGCCTCTAGC	63 °C
PLXNB2	TACGATGCCTTCCTGACCTC	TCTTGGAACTGCTCCCAGTC	61 °C
AXIN2	TGCTTTCGTGGAAATGACAG	AGGTGTGTGGAGGAAAGGTG	61 °C
GAPDH	ATGTTCGTCATGGGTGTGAA	GTCTTCTGGGTGGCAGTGAT	59–63 °C