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. 2013 May 9;108(10):2056–2062. doi: 10.1038/bjc.2013.200

Figure 2.

Figure 2

Functional characterisation of ARID1B permanent transfectants of MiaPaCa2. Panels (A) and (B) show result of MTT and crystal violet dye extraction growth assays performed for the two ARID1B (A3 and A8) and two vector (PC14 and PC15) clones of MiaPaCa2, respectively. Error bars represent s.e.m. of two (MTT assay) and three (crystal violet assay) independent experiments (performed in duplicate), respectively. Panels (C) and (E) show representative results of liquid and soft agar colony-formation assays, respectively. Panels (D) and (F) show graphical representation of result of liquid and soft agar colony-formation assays, respectively. Panel (G) shows quantitation of β-galactosidase staining in the same clones. Error bars represent s.e.m. of three independent liquid colony formation (in duplicate), soft agar and β-galactosidase staining (both in triplicate) experiments. P-value corresponds to unpaired Student's t-test.