Dkk2-1S
|
5′-AAACTAGCGGCCGCTAGTtgtccaatatatGTGGGAaT-3′
|
Dkk2-1AS
|
5′-CTAGAtTCCCACatatattggacaACTAGCGGCCGCTAGTTT-3′
|
sFRP1-1S
|
5′-AAACTAGCGGCCGCTAGTcctcgGGTGGGGTGGGAgT-3′
|
sFRP1-1AS
|
5′-CTAGAcTCCCACCCCACCcgaggACTAGCGGCCGCTAGTTT-3′
|
sFRP1-2S
|
5′-AAACTAGCGGCCGCTAGTgctagGGCCAAGGTGGGAtT-3′
|
sFRP1-2AS
|
5′-CTAGAaTCCCACCTTGGCCctagcACTAGCGGCCGCTAGTTT-3′
|
Smad4-1S
|
5′-AAACTAGCGGCCGCTAGTccGGGAGTAGATCGTGGGAtT-3′
|
Smad4-1AS
|
5′-CTAGAaTCCCACGATCTACTCCCggACTAGCGGCCGCTAGTTT-3′
|
sFRP1 NheI cloning forward primer
|
5′-GCTAGCgaggtccctggaagtttgc-3′
|
sFRP1 XhoI cloning reverse primer
|
5′-CTCGAGcgactggattacaatgtccactac-3′
|
Smad4 NheI cloning forward primer
|
5′-GCTAGCttgcttcagaaattggagacatatt-3′
|
| Smad4 XhoI cloning reverse primer |
5′-CTCGAGattttgtagtccaccatcctgataa-3′ |
