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. 2013 May 7;108(10):2045–2055. doi: 10.1038/bjc.2013.214

Table 3. Reactivity of discovery set and validation set sera from women who subsequently developed breast cancer (cases) and controls.

MUC1 peptide/glycopeptide antigen Samples Q1 Q2 Q3 Q4
(A) Discovery set
MUC1 peptide unglycosylated (60mer) Cases 66 70 61 76
  Controls 70 67 75 61
 
P-value
0·732
0·798
0·230
0·200
MUC1core3 glycopeptide (60mer) Cases 63 63 66 81
  Controls 73 73 71 56
 
P-value
0·391
0·391
0·670
0·0327
MUC1STn glycopeptide (60mer) Cases 67 68 60 78
  Controls 69 68 77 59
 
P-value
0·864
1
0·146
0·105
MUC1Tn glycopeptides (60mer) Cases 62 60 69 82
  Controls 74 77 67 55
 
P-value
0·303
0·146
0·864
0·0210
MUC1T glycopeptides (60mer) Cases 69 60 70 74
  Controls 67 77 66 63
 
P-value
0·864
0·146
0·732
0·347
MUC1T CHO recombinant (16 tandem repeats) Cases 64 68 68 73
  Controls 72 69 68 64
 
P-value
0·492
0·932
1
0·442
MUC1Tn CHO recombinant (16 tandem repeats) Cases 58 70 70 75
  Controls 78 67 66 62
 
P-value
0·086
0·798
0·732
0·267
MUC1ST CHO recombinant (16 tandem repeats) Cases 62 61 73 77
  Controls 74 75 64 60
 
P-value
0·303
0·230
0·442
0·146
MUC2core3 glycopeptide Cases 61 71 71 70
  Controls 74 67 65 65
 
P-value
0·263
0·733
0·607
0·798
(B) Validation set
UKCTOCS cohort
 
 
 
 
 
MUC1 peptide unglycosylated Cases 109 108 106 103
(60mer) Controls 104 105 107 110
 
P-value
0·732
0·837
0·945
0·631
MUC1core3 glycopeptides (60mer) Cases 108 97 114 107
  Controls 105 116 99 106
 
P-value
0·837
0·193
0·304
0·945
MUC1STn glycopeptides (60mer) Cases 103 108 110 105
  Controls 110 105 103 108
 
P-value
0·631
0·837
0·631
0·837
MUC1Tn glycopeptides (60mer) Cases 115 93 105 113
  Controls 98 120 108 100
 
P-value
0·244
0·064
0·837
0·373
MUC1T CHO recombinant Cases 105 113 96 112
(16 tandem repeats) Controls 108 100 117 101
 
P-value
0·837
0·373
0·150
0·451
MUC1Tn CHO recombinant Cases 103 103 107 113
(16 tandem repeats) Controls 110 110 106 100
 
P-value
0·631
0·631
0·945
0·373
MUC1ST CHO recombinant Cases 115 106 98 107
(16 tandem repeats) Controls 98 107 115 106
  P-value 0·244 0·945 0·244 0·945
Guernsey cohort
MUC1 peptide unglycosylated Cases 74 66 78 85
(60mer) Control 1 77 69 86 71
  Control 2 76 92 64 71
 
P-value
0·806
0·796
0·532
0·262
MUC1core3 glycopeptides (60mer) Cases 77 68 84 74
  Control 1 78 79 76 70
  Control 2 72 80 68 83
 
P-value
0·936
0·364
0·527
0·739
MUC1STn glycopeptides (60mer) Cases 79 78 72 74
  Control 1 72 78 77 76
  Control 2 76 71 79 77
 
P-value
0·569
1
0·682
0·870
MUC1Tn glycopeptides (60mer) Cases 73 72 76 82
  Control 1 81 79 79 64
  Control 2 73 76 73 81
 
P-value
0·519
0·569
0·809
0·136
MUC1T CHO recombinant (16 tandem repeats) Cases 83 74 71 75
  Control 1 70 80 74 79
  Control 2 74 73 83 73
 
P-value
0·293
0·629
0·803
0·240
MUC1Tn CHO recombinant (16 tandem repeats) Cases 84 75 66 78
  Control 1 75 82 82 64
  Control 2 68 70 80 85
 
P-value
0·475
0·576
0·188
0·240
MUC1ST CHO recombinant (16 tandem repeats) Cases 78 81 74 70
  Control 1 75 70 71 87
  Control 2 74 76 83 70
  P-value 0·808 0·370 0·803 0·175

Abbreviation: UKCTOCS=UK Collaborative Trial of Ovarian Cancer Screening.

Cases and controls were divided into quartiles dependent on the reactivity of their sera with the indicated antigens.