Figure 1.

Study design: 220 CRC patients were entered into the study. Cases were reviewed for clinicopathological features and characterised for B-Raf, KRAS mutations and MMR protein expression. Immunohistochemistry for RKIP was performed on full-tissue sections. Geographic analysis of RKIP expression patterns was performed in 10HPF each of tumour centre, tumour front and tumour buds as well as normal mucosa (5 HPF). Expression of E-Cadherin and NF-κB was assessed on a matched multi-punch TMA. The association of RKIP protein expression with prognostic features, tumour morphology, survival time, markers of EMT and molecular characteristics was carried out for each histological zone.