Table 3. Detection of rare BRAF mutations with different methods.

Patient ID	Pyrosequencing/Sanger sequencing	COBAS	Immunohistochemistry BRAF V600E	Localisation/organ
1	V600K	√	×	Brain
	V600K	ND	ND	uk
2	V600EK601del	×	ND	Primary tumour
	V600EK601del	ND	√	Stomach
	V600EK601del	ND	√	Pancreas
3	V600D	×	ND	Lymph node
	V600D	ND	×	Lung
	V600D	×	ND	uk
4	V600G	×	ND	Liver
5	V600K	√	ND	uk
	V600K	√	×	Lymph node
6	V600K	√	ND	uk
7	L597S	×	ND	uk
	L597S	ND	×
	L597S	ND	ND	Lymph node
8	V600E2 (GAA)	Invalid	√	Primary tumour
	V600E2 (GAA)	×	√	Lymph node
9	V600DK601del	√	×	Skin
	V600DK601del	ND	×	Skin
10	V600R	ND	×	Skin
	V600R	ND	×	Skin
	V600R	ND	×	Skin
	V600R	×	×	Skin
	V600R	×	×	Skin
11	V600K	√	ND	Skin
12	V600K	Invalid	ND	Skin
	V600K	ND	×	Skin
	V600K	√	ND	Lung
	V600K	ND	×	Skin
	V600K	Invalid	ND	Skin
13	V600K	√	ND	Skin
	V600K	ND	√	Skin
14	V600E2 (GAA)	×	ND	Skin
	V600E2 (GAA)	×	ND	Skin

Abbreviations: ND=not done; √=mutation detected; × =mutation not detected; Invalid=no result; uk=unknown.