Figure 4.

Flow cytometric analysis of Sox11 and nestin expression in glioblastoma cell cultures. Unstained glioblastoma cells were used for gating (R1) of the cell population for analysis (A). Single staining of nestin (B, D) and Sox11 (C, E) expression is shown in histograms and dot plots, respectively. Isotype-specific negative control is depicted in green. Double staining of glioma cells for nestin and Sox11 revealed a subpopulation of 9.14% of gated cells being positive for both proteins (F). The bar graph presents the fraction of positive cells (mean values) for Sox11 and/or nestin staining in the three primary glioblastoma culture (G).