Table 2. Characteristics of primary antibodies used in IHC and FACS.

Protein	Clone	Company	Catalogue no.	Raised in	Positive controls	No of stained slides for IHC	Antigen retrieval method for IHC	Dilution and incubation time for IHC	Dilution and incubation time for FACS
SOX11	Polyclonal	Spring, Pleasanton, CA, USA	110401A	Rabbit	Mantle cell lymphoma	132	Citrate buffer, pH 9	1 : 1000, 1 h (room temperature)	1 : 100a, 16 h (4 °C)
c-Met	Polyclonal	Invitrogen, Greece	182257	Rabbit	Breast carcinoma	111	Citrate buffer, pH 6	1 : 200, 18 h (4 °C)	—
Nestin	Monoclonal (10c2)	Santa Cruz, CA, USA	Sc-23927	Mouse	Kidney	92	Citrate buffer, pH 6	1 : 100. 1 h, (room temperature)	1 : 50b, 16 h (4 °C)
IDH1-R132H	monoclonal (clone H09)	Dianova, Hamburg, Germany	DIA H09	Mouse	Oligodendroglioma, diffuse astrocytoma	111	Citrate buffer, pH 6	1 : 50, 18 h (4 °C)	—
p-STAT-3 (specific for Tyr 705)	Monoclonal (clone (D3A7)	Cell Signaling, Danvers, MA, USA	D3A7 XP	Rabbit	Breast carcinoma	113	Citrate buffer, pH 6	1 : 50, 18 h (4 °C)	—

Abbreviations: FACS=flow cytometric analysis; FITC=fluorescein isothiocyanate; PE=phycoerythrin; p-STAT-3=signal transducer and activator of transcription.

^aIncubation with secondary antibody donkey anti-rabbit-PE (558416, BD Biosciences) at dilution 1 : 200 for 1 h at room temperature.

^bIncubation with secondary antibody goat anti-mouse-FITC (sc-2010, Santa Cruz Biotechnology) at dilution 1 : 200 for 1 h at room temperature.