Figure 3.

Propolis effect in vitro on L. braziliensis phagocytosis by macrophages. Monolayers of macrophages were treated with propolis (5 or 10 μg/mL) or with RPMI culture medium (control), incubated for 24 h at 37°C, and then coincubated for 2 h with promastigotes forms. (a) Percentage of macrophages phagocytosis; (b) number of intracellular amastigotes per macrophage after 2 h; (c) kinetics of recovered promastigotes from infected macrophages. Data represent mean ± SEM of three independent experiments. *Significantly different from control (P < 0.05).