Table 2.
Genomic Mapping of Selected Insertion Lines
| Tübingen driver line # | Expression pattern: Stage | Insertion position (LG:bp), notes |
|---|---|---|
| 6 | Primary motorneurons: 24 hpf | * |
| 13 | Muscle fibers, skin, vasculature, and unidentified cell types: 3 dpf | 23:24,886,350 intergenic, in promoter region of kans12; mutant phenotype linked to GFP expression |
| 22 | Scales, adult | |
| 40 | Trunk skeletal muscle fibers: 48 hpf | * |
| 42 | Myogenic lineage: 48 hpf | * |
| 45-1 | Epidermis: 24 hpf | * |
| 45-2 | Liver, pancreas: 4 dpf | 24:25,590,869 5′UTR of zgc:92111 (osta) |
| 64 | Trunk skeletal muscle fibers, strongest labeling in posterior of larva: 48 hpf | 18:20,649,309 exonic within synemin |
| 74 | Pectoral fin, adult | |
| 91 | Presomitic mesoderm, later in skeletal muscle in posterior of larva | 10:10,933,328 intronic of si:ch211-183l21.3 |
| 118-1 | Cells surrounding notochord (epithelial-like hexagonal shape): 48 hpf | * |
| 137 | Spinal cord neurons: 24 hpf | 10:7,973,728 intronic of LOC564899 |
| 200 | Skin, trunk skeletal muscles, notochord | 7:8,217,106 exonic within zgc:101810 |
| 201 | Cranial neural crest, branchial arches, ear: 48 hpf | 5: 16,930,847 intronic of gnb1l |
| 206 | Cranial neural crest, myogenic lineage: 24 hpf | 21:44,248,727 intergenic |
| 220-1 | Dorsal slow muscle fibers, posterior retina: 48 hpf | 9:16,204,267 intronic of si:dkey-114e9 |
| 234 | Midbrain, 24 hpf | * |
| 235-1 | CNS neurons, 24 hpf | 13:7,424,690 intronic of col13a1 |
| 237 | Trunk skeletal muscle fibers, skin, gut (strongest in posterior of larva), and pectoral fins: 48 hpf | * |
| 244-1 | Epidermis: 24 hpf | 1:34,215,682 intergenic |
| 244-3 | Vasculature: 72 hpf | * |
| 244-4 | Pectoral fins, heart valves, dorsal retina: 48 hpf | * |
| 245 | CNS neurons: 24 hpf | |
| 275 | Myogenic lineage: 48 hpf | 16:33,668,562 intergenic |
| 283 | Myogenic lineage: 24 hpf | 13:23,419,906 intronic of si:dkey-103j14.2 |
| 296-1 | Slow skeletal muscles, dermal ionocytes: 48 hpf | * |
| 296-2 | Finfold edges: 48 hpf | 5:28,460,264 intronic of si:ch211-102c2.5 |
| 296-3 | Erythrocytes: 24 hpf | 3:55,961,349 intergenic |
| 302 | Liver, pancreas: 6 dpf | 3:25,723,273 intronic of LOC100149276 (usp43) |
| 318 | Lateral line neuromasts, ENS, jaw, pharynx, tectum: 48 hpf | 25:19,091,711 intronic of met4:∼25,248, 000-25,379,000 intergenic |
| 354-1 | CNS neurons: 24 hpf | * |
| 358 | Iridophores, lateral line glia: 72 hpf Maternal/ubiquitous expression earlier |
* |
Selected lines with specific expression and minimally variegating patterns were chosen for genomic mapping of insertion sites using TAIL PCR. Some mappings resulted in a lack of clear TAIL PCR product (*). The stage in hpf (hours post fertilization) indicates the time point when the GFP expression pattern is fully developed, although GFP expression can start earlier.