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. Author manuscript; available in PMC: 2013 Jun 3.
Published in final edited form as: J Immunol. 2010 Dec 27;186(3):1369–1376. doi: 10.4049/jimmunol.1002047

Figure 6.

Figure 6

IL-6 mRNA expression is up regulated in the lung and liver of Dragon knockout mice and embryos. (A-C) Total RNA was extracted from lungs (A) and livers (C) of wild type (WT) and Dragon knockout (KO) mice at ages of day 10–12 or from lungs (B) of E20.5 wild type and Dragon fetuses. mRNA levels were measured by quantitative real-time PCR, were normalized to RPL19 mRNA levels, and are expressed as a fraction of values from WT samples. (D) IL-6 protein is up regulated in the lung of Dragon knockout mice. Lysates from lungs of 10–12 days old wild type (+/+) and Dragon knockout (−/−) mice were subjected to Western blotting for IL-6. The membrane was stripped and probed with Actin antibodies. (E) Changes in phosphorylation levels of Smad1/5/8, and p38 and Erk1/2 MAPK in Dragon knockout lungs. Lysates from lungs of 10–12 days old wild type (+/+) and Dragon knockout (−/−) mice were subjected to Western blotting for phospho-Smad1/5/8, phospho-p38 MAPK and phospho-Erk1/2. The membranes were stripped and probed with total Smad1, p38-MAPK and Erk1/2 antibodies respectively. *, p < 0.05; **, p < 0.01; *** p < 0.001 versus wild type. n=6–7.