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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Anal Chim Acta. 2013 Feb 24;772:93–98. doi: 10.1016/j.aca.2013.02.023

Table 4.

Reproducibility of protein migration times as function of time.

Mean migration time (min) (n =3)
Capillary Day 10 Day 30 Day 45
His-EGFP RPE His-EGFP RPE His-EGFP RPE
1 1.8 (0.16) 2.4 (0.08) 1.8 (0.04) 2.37 (0.23) 1.8 (1.25) 2.4 (1.80)
2 1.8 (0.37) 2.4 (0.26) 1.8 (0.47) 2.30 (0.42) 1.7 (0.29) 2.3 (0.13)
3 1.8 (0.08) 2.4 (0.09) 1.8 (0.24) 2.34 (0.26) 1.7 (0.62) 2.3 (0.69)

Conditions: Capillaries, 42 cm × 50 μm i.d., 32 cm to detector; E = 240 V/cm; 20 mM borate buffer at pH 9.3. Coumarin 334 was used as EOF marker. The values in bracket are %RSD.