Table 1.
Effects of TMZ and nNOS I on mitochondrial respiration
| Substrates | Experiments | State 2 | State 3 | RCR |
|---|---|---|---|---|
|
| ||||
| Complex I | cntrl (n=6) | 78.3±8.9 | 493.8±0.03 | 6.62±0.78 |
| cntrl + TMZ (n=6) | 77.3±7.5 | 409.7±0.04 | 5.61±0.92 | |
| cntrl + nNOS I (n=6) | 60.5±7.4# | 449.9±4.94 | 7.86±0.58# | |
|
| ||||
| HF (n=8) | 56.0±7.8* | 136.5±26.6* | 2.43±0.21* | |
| HF + TMZ (n=9) | 93.7±7.3# | 243.1±17.3# | 2.59±0.32 | |
| HF + nNOS I (n=8) | 75.2±3.9# | 189.7±9.4# | 2.52±0.18 | |
|
| ||||
| Complex II | cntrl (n=6) | 230.2±32.1 | 548.5±27.5 | 2.53±0.22 |
| cntrl + TMZ (n=6) | 275.7±31.2 | 558.7±43.6 | 2.13±0.19 | |
| cntrl + nNOS I (n=6) | 235.1±21.6 | 500.3±26.6 | 2.13±0.18 | |
|
| ||||
| HF (n=5) | 428.6±51.7* | 584.0±34.3 | 1.50±0.28* | |
| HF + TMZ (n=5) | 329.4±65.9# | 356.4±62.6# | 1.54±0.58 | |
| HF + nNOS I (n=5) | 424.1±49.3 | 552.4±46.5 | 1.30±0.59 | |
|
| ||||
| Palmitoyl-carnitine | cntrl (n=4) | 124±6.3 | 522.0±52.0 | 4.19±0.38 |
| cntrl + TMZ (n=4) | 106±2.5# | 405.7±27.3# | 3.84±0.25 | |
| cntrl + 4-BA (n=4) | 102.9±6.9# | 391.5±58.4# | 3.80±0.21 | |
State 2 and state 3: rates of O2 consumption, nmol O2/min per 105 cells
RCR: state 3 respiration/state 2 respiration
*
P<0.05, HF compared to control cells
#
P<0.05, TMZ or nNOS I treated cells compared to cells in the absence of the drug