Table 1.
Identified H2AX-interacting Proteins in HCC QGY7703 Cells.
| IPI no. | Gene symbol | Protein namea | No.of peptide-matched | Enrichment-foldb | S.D. |
|---|---|---|---|---|---|
| IPI00219037.5 | H2AX | Histone H2AX (bait) | 4 | 9.11 | |
| Apoptosis | |||||
| IPI00012011.6 | CFL1 | Cofilin-1 | 2 | 1.90 | 0.014 |
| IPI00291262.3 | CLU | Clusterin precursor | 2 | 2.26 | 0.56 |
| IPI00027230.3 | HSP90B1 | Endoplasmin precursor | 8 | 1.60 | 0.07 |
| IPI00304925.4 | HSPA1A | Heat shock 70 kDa protein 1 | 3 | 1.50 | 0.16 |
| IPI00555692.2 | ANXA4 | ANXA4 protein | 2 | 1.49 | 0.72 |
| IPI00329801.12 | ANXA5 | Annexin A5 | 6 | 1.36 | 0.11 |
| IPI00025277.5 | PDCD6 | Programmed cell death protein 6 | 3 | 1.745 | 0.19 |
| IPI00171438.2 | TXNDC | Thioredoxin domain-containing protein 5 precursor | 2 | 1.62 | 0.08 |
| IPI00291467.7 | SLC25A6 | ADP/ATP translocase 3 | 2 | 1.72 | 0.057 |
| Response to protein stimulus, oxidative stress and hypoxia | |||||
| IPI00023137.1 | TOR1B | Torsin B precursor | 2 | 2.71 | 0.70 |
| IPI00382470.3 | HSP90AA1 | Heat shock protein HSP 90-alpha 2 | 3 | 2.57 | 0.19 |
| IPI00301277.1 | HSPA1L | Heat shock 70 kDa protein 1L | 3 | 1.45 | 0.13 |
| IPI00030275.5 | TRAP1 | Heat shock protein 75 kDa mitochondrial precursor | 9 | 2.45 | 0.24 |
| IPI00220301.5 | PRDX6 | Peroxiredoxin-6 | 3 | 1.73 | 0.38 |
| IPI00027192.5 | PLOD1 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase precursor | 2 | 2.46 | 0.03 |
| IPI00022314.1 | SOD2 | Superoxide dismutase90, | 2 | 2.56 | 0.22 |
| DNA repair (14) | |||||
| IPI00000690.1 | AIFM1 | Isoform 1 of Apoptosis-inducing factor 1, mitochondrial precursor | 6 | 2.93 | 0.37 |
| IPI00304596.3 | NONO | Non-POU domain-containing octamer-binding protein | 2 | 1.93 | 0.02 |
| IPI00449049.5 | PARP1 | Poly [ADP-ribose] polymerase 1 | 2 | 1.81 | 0.07 |
| IPI00020599.1 | CALR | Calreticulin precursor | 7 | 1.34 | 0.20 |
| IPI00021263.3 | YWHAZ | 14-3-3 protein zeta/delta | 3 | 1.37 | 0.11 |
| IPI00000816.1 | YWHAE | 14-3-3 protein epsilon | 3 | 1.71 | 0.10 |
| IPI00413293.5 | TOR1A | Torsin A precursor | 2 | 1.81 | 0.56 |
| IPI00000877.1 | HYOU1 | 150 kDa oxygen-regulated protein precursor | 4 | 2.68 | 0.28 |
| IPI00029744.1 | SSBP1 | Single-stranded DNA-binding protein, mitochondrial precursor | 2 | 1.74 | 1.12 |
| IPI00169383.3 | PGK1 | Phosphoglycerate kinase 1 | 4 | 2.58 | 0.61 |
| IPI00219365.3 | MSN | Moesin | 2 | 1.96 | 0.76 |
| IPI00025491.1 | EIF4A1 | Eukaryotic initiation factor 4A-I | 2 | 1.41 | 0.04 |
| IPI00008438.1 | RPS10 | 40S ribosomal protein S10 | 2 | 2.14 | 0.03 |
| IPI00011062.1 | CPS1 | Isoform 1 of Carbamoyl-phosphate synthase [ammonia], mitochondrial precursor | 4 | 1.44 | 0.05 |
| DNA metabolism | |||||
| IPI00005198.2 | ILF2 | Interleukin enhancer-binding factor 2 | 3 | 1.71 | 0.12 |
| IPI00453473.6 | HIST2H4A | Histone H4 | 5 | 2.27 | 0.44 |
| Ca2+ binding signaling (6) | |||||
| IPI00418169.3 | ANXA2 | annexin A2 isoform 1 | 3 | 1.67 | 0.27 |
| IPI00334627.3 | ANXA2P2 | Similar to annexin A2 isoform 1 | 6 | 1.84 | 0.20 |
| IPI00075248.11 | CALM2 | Calmodulin | 6 | 5.35 | 0.97 |
| IPI00032313.1 | S100A4 | Protein S100-A4 | 2 | 1.87 | 0.01 |
| IPI00018235.3 | PEF1 | Peflin | 2 | 3.08 | 0.54 |
| IPI00002459.4 | ANXA6 | annexin VI isoform 2 | 8 | 2.19 | 0.22 |
| Cell cycle | |||||
| IPI00216318.5 | YWHAB | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide | 2 | 1.48 | 0.04 |
| IPI00017297.1 | MATR3 | Matrin-3 | 2 | 2.44 | 0.02 |
| Alcohol metabolic process | |||||
| IPI00465439.5 | ALDOA | Fructose-bisphosphate aldolase A | 2 | 2.015 | 0.049 |
| IPI00220644.8 | PKM2 | pyruvate kinase 3 isoform 2 | 6 | 2.45 | 0.27 |
| IPI00479186.5 | PKM2 | pyruvate kinase 3 isoform 1 | 2 | 2.34 | 0.17 |
| IPI00465248.5 | ENO1 | enolase 1 | 2 | 1.945 | 0.16 |
| IPI00058192.1 | POFUT1 | Isoform 1 of GDP-fucose protein O-fucosyltransferase 1 precursor | 3 | 1.86 | 0.05 |
| IPI00219217.3 | LDHB | L-lactate dehydrogenase B chain | 2 | 2.15 | 0.19 |
| RNA metabolism | |||||
| IPI00301936.3 | ELAVL1 | ELAV-like protein 1 | 3 | 1.47 | 0.22 |
| IPI00028888.1 | HNRPD | Isoform 1 of Heterogeneous nuclear ribonucleoprotein D0 | 3 | 1.39 | 0.49 |
| IPI00011274.2 | HNRPDL | heterogeneous nuclear ribonucleo protein D-like | 2 | 1.84 | 0.10 |
| IPI00013877.2 | HNRPH3 | Isoform 1 of Heterogeneous nuclear ribonucleoprotein H3 | 2 | 3.12 | 0.36 |
| IPI00012074.3 | HNRPR | Heterogeneous nuclear ribonucleoprotein R | 5 | 3.26 | 0.31 |
| IPI00106509.2 | HNRPAB | Isoform 4 of Heterogeneous nuclear ribonucleoprotein A/B | 2 | 4.57 | 0.13 |
| IPI00012066.2 | PCBP2 | poly(rC)-binding protein 2 isoform b | 2 | 1.86 | 0.77 |
| IPI00008524.1 | PABPC1 | Isoform 1 of Polyadenylate-binding protein 1 | 2 | 4.18 | 0.54 |
| IPI00221035.3 | BTF3 | Isoform 1 of Transcription factor BTF3 | 2 | 2.63 | 0.05 |
| IPI00073713.3 | MSI2 | Isoform 1 of RNA-binding protein Musashi homolog 2 | 2 | 2.81 | 0.65 |
| IPI00328840.9 | THOC4 | THO complex subunit 4 | 3 | 4.76 | 0.70 |
| IPI00018140.3 | SYNCRIP | Isoform 1 of Heterogeneous nuclear ribonucleoprotein Q | 9 | 2.62 | 0.35 |
| IPI00215965.2 | HNRPA1 | heterogeneous nuclear ribonucleoprotein A1 isoform b | 2 | 1.42 | 0.20 |
| IPI00386854.5 | HNRPA2B1 | HNRPA2B1 protein | 4 | 2.59 | 0.17 |
| IPI00216592.2 | HNRPC | Isoform C1 of Heterogeneous nuclear ribonucleoproteinsC1/C2 | 3 | 1.84 | 0.04 |
| Establishment of cellular localization | |||||
| IPI00018931.6 | VPS35 | Vacuolar protein sorting-associated protein35 | 3 | 1.845 | 0.15 |
| IPI00024911.1 | ERP29 | Endoplasmic reticulum protein ERp29 precursor | 5 | 1.49 | 0.16 |
| IPI00156689.3 | VAT1 | Synaptic vesicle membrane protein VAT-1 homolog | 3 | 2.27 | 0.45 |
| IPI00021405.3 | LMNA | Isoform A of Lamin-A/C | 3 | 1.53 | 0.06 |
| IPI00045839.3 | LEPRE1 | Isoform 3 of Prolyl 3-hydroxylase 1 precursor | 3 | 1.35 | 0.15 |
| IPI00009904.1 | PDIA4 | Protein disulfide-isomerase A4 precursor | 11 | 1.58 | 0.08 |
| IPI00020436.4 | RAB11B | Ras-related protein Rab-11B | 3 | 1.47 | 0.60 |
| IPI00023748.3 | NACA | Nascent polypeptide-associated complex subunit alpha | 2 | 1.50 | 0.63 |
| Cellular macromolecule metabolism | |||||
| IPI00021728.3 | EIF2S2 | Eukaryotic translation initiation factor 2 subunit 2 | 2 | 1.36 | 0.13 |
| IPI00178440.3 | EEF1B2 | Elongation factor 1-beta | 2 | 1.52 | 0.58 |
| IPI00299571.5 | PDIA6 | Isoform 2 of Protein disulfide-isomerase A6 precursor | 9 | 1.60 | 0.05 |
| IPI00010720.1 | CCT5 | T-complex protein 1 subunit epsilon | 2 | 1.57 | 012 |
| IPI00011229.1 | CTSD | Cathepsin D precursor | 3 | 3.61 | 0.39 |
| IPI00386755.2 | ERO1L | ERO1-like protein alpha precursor | 2 | 1.56 | 0.11 |
| IPI00010796.1 | P4HB | Protein disulfide-isomerase precursor | 6 | 1.47 | 0.42 |
| IPI00011937.1 | PRDX4 | Peroxiredoxin-4 | 3 | 2.19 | 0.27 |
| IPI00419585.9 | PPIA | Peptidyl-prolyl cistrans isomerase A | 4 | 1.44 | 0.06 |
| IPI00028004.2 | PSMB3 | Proteasome subunit beta type 3 | 2 | 2.26 | 0.74 |
| IPI00008529.1 | RPLP2 | 60S acidic ribosomal protein P2 | 2 | 1.40 | 0.01 |
| IPI00555956.2 | PSMB4 | Proteasome subunit beta type 4 precursor | 2 | 1.76 | 0.43 |
| IPI00303300.3 | FKBP10 | FK506-binding protein 10 precursor | 3 | 2.39 | 0.96 |
| IPI00171412.1 | SUMF2 | Isoform 1 of Sulfatase-modifying factor 2 precursor | 2 | 2.38 | 0.02 |
| IPI00003128.1 | P4HA2 | Isoform IIb of Prolyl 4-hydroxylase subunit alpha-2 precursor | 3 | 2.3 | 0.14 |
| IPI00028635.4 | RPN2 | Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 63 kDa subunit precursor | 7 | 2.20 | 0.02 |
| Cell redox homeostasis | |||||
| IPI00026328.3 | TXNDC12 | Thioredoxin domain-containing protein 12 precursor | 3 | 1.35 | 0.04 |
| IPI00303568.3 | PTGES2 | Prostaglandin E synthase 2 | 6 | 1.71 | 0.34 |
| Carboxylic acid metabolism | |||||
| IPI00017726.1 | HSD17B10 | hydroxysteroid (17-beta) dehydrogenase 10isoform 1 | 5 | 2.15 | 0.35 |
| IPI00643920.2 | TKT | Transketolase | 3 | 3.52 | 0.31 |
| Carbohydrate catabolism | |||||
| IPI00011454.1 | GANAB | Isoform 2 of Neutral alpha-glucosidase AB precursor | 3 | 1.44 | 0.42 |
| IPI00441344.1 | GLB1 | Beta-galactosidase precursor | 2 | 4.08 | 0.38 |
| IPI00383046.3 | CMBL | carboxymethylenebutenolidase-like | 7 | 4.15 | 0.29 |
| IPI00026154.2 | PRKCSH | Glucosidase 2 subunit beta precursor | 4 | 2.21 | 1.00 |
| IPI00023673.1 | LGALS3BP | Galectin-3-binding protein Precursor | 3 | 3.22 | 0.29 |
| IPI00027851.1 | HEXA | Beta-hexosaminidase alpha chain Precursor | 2 | 3.01 | 0.16 |
| Others | |||||
| IPI00020075.4 | ABHD10 | CDNA FLJ11342 fis, clone PLACE1010800 | 3 | 2.10 | 0.05 |
| IPI00168479.2 | APOA1BP | apolipoprotein A-I binding protein precursor | 3 | 3.36 | 0.22 |
| IPI00550363.3 | CCDC19 | Transgelin-2 | 3 | 1.44 | 0.67 |
| IPI00329696.1 | FAM82B | Protein FAM82B | 3 | 3.08 | 0.08 |
| IPI00007765.5 | HSPA9 | Stress-70 protein, mitochondrial precursor | 9 | 1.63 | 0.74 |
| IPI00024919.3 | PRDX3 | Thioredoxin-dependent peroxide reductase, mitochondrial precursor | 3 | 2.81 | 0.34 |
| IPI00377161.7 | HIBCH | 3-hydroxyisobutyryl-Coenzyme A hydrolase isoform 2 | 2 | 4.32 | 0.63 |
Only the proteins with two or more peptides matched are listed.
Fold of enrichment was calculated as the ratio of Leu-d3-labeled peptide to unlabeled peptide.