Table 1.

MRM transitions for 100-pg RAW 264.7 cell lysate digest analysis.

Q1 (m/z)	Q3 (m/z)	dwell time (ms)	protein description_peptide sequence.peptide charge	collision energy (eV)
661.68	884.94	30	Peroxiredo_TIAQDYGVLKADEGISFR.3	37.084
661.68	785.39	30	Peroxiredo_TIAQDYGVLKADEGISFR.3	37.084
613.35	770.42	30	Peroxiredo_QITINDLPVGR.2	31.987
613.35	984.55	30	Peroxiredo_QITINDLPVGR.2	31.987
400.24	441.27	30	Actin_AVFPSIVGRPR.3	24.012
400.24	881.53	30	Actin_AVFPSIVGRPR.3	24.012
488.73	630.28	30	Actin_AGFAGDDAPR.2	26.504
488.73	701.32	30	Actin_AGFAGDDAPR.2	26.504
728.38	773.45	30	Profilin-1_SSFFVN[Dea]GLTLGGQK.2	37.049
728.38	888.48	30	Profilin-1_SSFFVN[Dea]GLTLGGQK.2	37.049
437.78	561.34	30	Profilin-1_TLVLLMGK.2	24.262
437.78	660.41	30	Profilin-1_TLVLLMGK.2	24.262
473.75	549.27	30	Pyruvate kinase_VNLAMDVGK.2	25.845
473.75	620.31	30	Pyruvate kinase_VNLAMDVGK.2	25.845
420.77	559.36	30	Pyruvate kinase_APIIAVTR.2	23.514
420.77	672.44	30	Pyruvate kinase_APIIAVTR.2	23.514
589.32	624.31	30	Alpha-enol_MILPVGASSFR.2	30.93
589.32	820.43	30	Alpha-enol_MILPVGASSFR.2	30.93
450.28	614.39	30	Alpha-enol_TIAPALVSK.2	24.812
450.28	685.42	30	Alpha-enol_TIAPALVSK.2	24.812
568.31	761.42	30	Plastin_QFVTATDVVR.2	30.006
568.31	860.48	30	Plastin_QFVTATDVVR.2	30.006
751.88	845.4	30	Plastin_MINLSVPDTIDER.2	38.083
751.88	944.47	30	Plastin_MINLSVPDTIDER.2	38.083
513.31	685.4	30	Elongation factor_IGGIGTVPVGR.2	27.586
513.31	912.53	30	Elongation factor_IGGIGTVPVGR.2	27.586
468.91	652.32	30	Elongation factor_YYVTIIDAPGHR.3	27.446
468.91	765.4	30	Elongation factor_YYVTIIDAPGHR.3	27.446
595.33	660.39	30	14-3-3 protein_DSTLIMQLLR.2	31.195
595.33	773.47	30	14-3-3 protein_DSTLIMQLLR.2	31.195
774.86	876.41	30	14-3-3 protein_SVTEQGAELSNEER.2	39.094
774.86	1004.46	30	14-3-3 protein_SVTEQGAELSNEER.2	39.094
523.79	660.37	30	Macrophage migration_LLC[CAM]GLLSDR.2	28.047
523.79	820.4	30	Macrophage migration_LLC[CAM]GLLSDR.2	28.047
644.35	700.37	30	Macrophage migration_PMFIVNTNVPR.2	33.351
644.35	799.44	30	Macrophage migration_PMFIVNTNVPR.2	33.351