Figure 3.

Acetylated H4K8, a marker of transcriptional activation, is augmented in the NAc after Hdac3 deletion and CPP conditioning. A, In this figure, data were collected from mice killed after either saline or 5 mg/kg cocaine conditioning in a single CPP trial. A schematic of the experimental design illustrates how this was done (fully described in Materials and Methods). B, Representative 4× and 20× magnified images of cocaine-treated Hdac3^+/+ (top) and Hdac3^flox/flox (bottom) coronal hemisections immunostained with anti-H4K8Ac IgG illustrate an overall increase of H4K8Ac in the NAc after Hdac3 deletion. C, Quantification of anti-H4K8Ac IHC demonstrates that H4K8Ac is more abundant in Hdac3^flox/flox mice compared with Hdac3^+/+ littermates. Hdac3^flox/flox: saline, n = 6; cocaine, n = 8; Hdac3^+/+: saline, n = 5; cocaine, n = 5; p = 0.024. *p < 0.05.