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. 2013 Apr 10;33(15):6623–6632. doi: 10.1523/JNEUROSCI.4472-12.2013

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Copyright © 2013 the authors 0270-6474/13/336623-10$15.00/0

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Figure 4. — Changes in cocaine- and learning- and memory-associated mRNA abundances in the NAc concomitant with increased H4K8Ac. A, Gene expression was analyzed from tissue punches collected from the NAc of the same brains shown in Figure 3B in the area of focal deletion as confirmed by IHC. c-Fos mRNA was increased in both genotypes after CPP conditioning with 5 mg/kg cocaine relative to conditioning with saline. A significant effect of genotype was also found by two-way ANOVA, indicating that more c-Fos mRNA was present in the NAc of Hdac3^flox/flox mice relative to Hdac3^+/+ littermates. Hdac3^flox/flox: saline, n = 6; cocaine, n = 8; Hdac3^+/+: saline, n = 5; cocaine, n = 5. *Significantly different from Hdac3^+/+, p < 0.05. ^#significantly different from saline, p < 0.05. B, Nr4a2 mRNA levels from the same tissue punches used in Figure 4B were significantly enhanced in cocaine-conditioned, but not saline-conditioned, Hdac3^flox/flox mice relative to Hdac3^+/+ littermates. There was a significant interaction between treatment and genotype affecting Nr4a2 mRNA abundance in the NAc. Hdac3^flox/flox: saline, n = 6; cocaine, n = 8; Hdac3^+/+: saline, n = 5; cocaine, n = 5. ^#Significantly different from saline, p < 0.05. ^&Significantly different from cocaine-conditioned Hdac3^+/+, p < 0.05. C, Grin3a mRNA levels from the same tissue punches were likewise significantly enhanced in cocaine-conditioned, but not saline-conditioned, Hdac3^flox/flox mice relative to Hdac3^+/+ littermates. Hdac3^flox/flox: saline, n = 6; cocaine, n = 8; Hdac3^+/+: saline, n = 5; cocaine, n = 5. &Significantly different from cocaine-conditioned Hdac3^+/+, p = 0.039. D, E, Egr2 and Hdac4 mRNA levels remained unchanged by treatment or genotype. Hdac3^flox/flox: saline, n = 6; cocaine, n = 8; Hdac3^+/+: saline, n = 5; cocaine, n = 5. F, Hdac5 mRNA is decreased in both genotypes after CPP conditioning with cocaine relative to conditioning with saline. A significant effect of genotype is also observed, indicating that less Hdac5 mRNA is present in the NAc of Hdac3^flox/flox mice relative to Hdac3^+/+ littermates. Hdac3^flox/flox: saline, n = 6 mice; cocaine, n = 8 mice; Hdac3^+/+: saline, n = 5; cocaine, n = 5. *Significantly different from Hdac3^+/+, p < 0.05. ^#Significantly different from saline, p < 0.05.