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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Biochemistry. 2013 Mar 19;52(13):2280–2290. doi: 10.1021/bi400097z

Fig. 2.

Fig. 2

Metal dependence of LpxK activity. (A) Purified LpxK, which was first incubated with EDTA, was then diluted into assay buffer containing 2 mM ATP and the indicated cation, and reaction was quenched by spotting on a TLC plate after 8 minutes. (B) The standard assay was performed in the presence of 5 mM ATP, and Mg2+ ranging from 0.015 to 128 mM. The cation is necessary for activity, but becomes inhibitory at high concentrations with respect to ATP.