Table 2.
Kinetic parameters of LpxK point mutants with respect to ATP/Mg2+
| LpxK Mutant | App. KM (mM) | Fold KM increase |
App. kcat (sec−1) | Fold kcat decrease |
|---|---|---|---|---|
| WT | 1.2 ± 0.2 | 1.0 | 3.9 ± 0.2 | 1.0 |
| S49A | 2.7 ± 0.3 | 2.3 | 3.9 ± 0.1 | 1.0 |
| K51A | 1.5 ± 0.4 | 1.3 | (1.3 ± 0.1)×10−3 | 3000 |
| T52A | 2.2 ± 0.3 | 1.8 | (1.3 ± 0.1)×10−3 | 3000 |
| S53A | 6 ± 1 | 5 | (4.7 ± 0.6)×10−1 | 8.3 |
| Y74A | 1.7 ± 0.3 | 1.4 | (2.2 ± 0.1)×10−2 | 180 |
| D99A | 2.8 ± 0.5 | 2.3 | (1.5 ± 0.1)×10−3 | 2600 |
| D99N | 1.1 ± 0.2 | 1.0 | (3.5 ± 0.2)×10−4 | 11000 |
| D99E | 2.3 ± 0.2 | 1.9 | (5.0 ± 0.2)×10−2 | 78 |
| E100A | 1.9 ± 0.5 | 1.6 | (2.9 ± 0.2)×10−3 | 1300 |
| E100Q | 2.9 ± 0.4 | 2.4 | (4.8 ± 0.2)×10−4 | 8100 |
| E100D | 2.0 ± 0.3 | 1.7 | (2.7 ± 0.1)×10−3 | 1400 |
| D138A | 4.0 ± 0.6 | 3.3 | (8.3 ± 0.4)×10−4 | 4700 |
| D138N | 3.2 ± 0.7 | 2.7 | (5.7 ± 0.5)×10−2 | 68 |
| D139A | 5 ± 1 | 4 | (4.8 ± 0.5)×10−4 | 8100 |
| D139N | 1.4 ± 0.3 | 1.2 | (7.6 ± 0.5)×10−3 | 510 |
| D260A | 1.6 ± 0.3 | 1.3 | (7.3 ± 0.4)×10−2 | 53 |
| H261A | 1.3 ± 0.2 | 1.1 | (4.6 ± 0.2)×10−3 | 850 |