Fig. 1.

Quantitative Real-time PCR (qRT-PCR) validation of the LEPR genes by Taqman Low Density Array (TLDA) in ABI platform (7900HT Fast Real-Time PCR System) after analysis by SDS RQ Manager Version 1.2.1. The relative quantification is calculated in contrast to calibrator samples, i.e.; the subjects with no /or background PCBs exposures in the population studies. The data are attenuated automatically and the reflection of triplicate observation already seated within the TLDA plates. This study represents that this high-throughput biomarker-based method will be capable of identifying high-risk individuals with specificity & selectivity, through therapeutically relevant genomic classifiers, as a measure of biological responses to environmental stressors.