TERT promoter and putative regulatory element (PRE) activity. Luciferase reporter assays following transient transfection of ER-negative breast cancer cell line (MDA-MB-468), ER-positive breast cancer cell line (MCF7), and ovarian cancer cell line (A2780). Error bars represent standard error between at least three separate experiments. (a-c) Luciferase reporter assays following transient transfection with pGL2-control (SV40 promoter and enhancer), pGL2-basic (lacks promoter and enhancer), and the TERT reporter vectors TERT wildtype (wt) (3.9kb of TERT promoter), the minor (T) alleles of rs2736107, rs2736108, rs2736109, and rs2736107/8/9 (T-alleles at all sites). Comparisons with TERT wt performed using one-way ANOVA with post hoc Dunnett's tests are represented by ** (P-value <0.001) and * (P-value 0.005).
(d–f) PRE-A or PRE-B were cloned downstream of either the TERT wt or TERT rs2736107/8/9 (TERTh) promoter-driven reporters with and without SNPs rs10069690, rs2242652 and rs7705526. Comparisons with TERT wt or TERTh performed using one way ANOVA with post hoc Dunnett's tests are represented by *** (P-value <0.0001) and ** (P-value <0.001).