. Author manuscript; available in PMC: 2013 Jun 3.

Published in final edited form as: J Am Chem Soc. 2011 Jul 22;133(32):12749–12757. doi: 10.1021/ja204005r

Table I.

Energy and kinetic parameters of GCN4 coiled coils measured by pulling from different directions.

	Pulling ends	Contour length (nm)	Equilibrium force (pN)	Equilibrium rate (1/s)	Transition state^* (nm)	Unfolding energy (k_BT)	Rate (1/s)^†
	Pulling ends	Contour length (nm)	Equilibrium force (pN)	Equilibrium rate (1/s)	Transition state^* (nm)	Unfolding energy (k_BT)	Folding	Unfolding
GCN4-p1C	N	25.8 (0.8)	7.5 (0.2)	171 (43)	16.2 (0.8)	12.9 (0.7)	3.7(0.8)×10⁵	0.9 (0.7)
GCN4-p1C’	N	17 (1)	6.6 (0.7)	359 (94)	10 (1)	6.6 (0.6)	2 (1)×10⁴	25 (22)
GCN4-p1N	C	23.8 (0.7)	6.3 (0.3)	6 (2)	17.9 (0.9)	8.6 (0.6)	3 (1)×10³	0.6 (0.4)
GCN4-p1Nc	N & C	7.8 (0.6)	12.1 (0.2)	~0.3	N.A.	8.5 (0.4)	N.A.	N.A.

The contour length distance to the unfolded state.

^†

At zero force