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. 2013 Jun 3;8(6):e66439. doi: 10.1371/journal.pone.0066439

Figure 4. Depletion of km23-1 inhibits cell migration and invasion of human CRC cells.

Figure 4

A: Transwell migration assays were performed as described in “Materials and methods.” Briefly, HCT116 cells stably transduced with lentiviral vectors expressing either piLenti-NC siRNA-GFP or piLenti-km23-1 siRNA-GFP were seeded into the upper wells of the Costar Transwell System (8-µm pore size polycarbonate membrane, 6.5-mm diameter), and the cells on the lower surface of the well after 24 h were fixed in methanol and stained with DAPI. Top, representative images of the lower surface of the membrane are shown (100× magnification). Bottom, the number of migrating cells of both NC siRNA- and km23-1-siRNA-HCT116 stable transfectants were counted under a fluorescence microscope and statistically analyzed. *p<0.01 compared to NC siRNA. B: Matrigel invasion assays were performed with the indicated RKO stable cells clones (clones #1, 5) for 24 h using EGF (20 ng/ml) as the stimulus as described in “Materials and methods.” Invaded cells were stained with 0.2% crystal violet. Top, representative images of the lower membrane surface from Matrigel are shown (100× magnification). Bottom, the number of invading cells for both NC siRNA and km23-1-siRNA HCT116 stable transfectants were counted under a light microscope and statistically analyzed. *p<0.01 compared to EV.