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Neutrophils were treated as indicated, lysed, and PI3-kinase was immunoprecipitated. Immunoprecipitated PI3-kinase was incubated with PIP[(4], [5)]2 substrate and the amount of resultant PIP3 was measured by ELISA. Graph represents the mean ± SEM of 3 independent experiments ( ** P<0.01, *** P<0.001, unpaired t test).
