Figure 5. PTEN inhibition restores Msp mediated impairment of actin uncapping.

A) Partially permeabilized neutrophils were treated with VO-OHpic prior to Msp treatment followed by fMLP stimulation. The amount of gelsolin and capZ released from actin filaments was quantified in collected supernatants and cell lysates by immunoblotting. Representative immunoblots are shown. B) Graphs of densitometry analysis of immunoblots represent mean ± SEM (n = 3, * P<0.05, ** P<0.01, *** P<0.001, unpaired t test).