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. 2013 Jun 3;8(6):e66209. doi: 10.1371/journal.pone.0066209

Figure 7. Rescue of active Rac1 is able to restore Msp mediated defects in actin uncapping.

Figure 7

TAT-mediated transduction of active Rac1 (Rac1CA) was able to rescue Msp-mediated defective gelsolin uncapping in response to fMLP stimulation. Cells were partially permeabilized followed by protein transduction with either 500 nM Rac1CA-TAT protein or TAT protein as a control, prior to Msp treatment and fMLP stimulation. A) A representative immunoblot showing the amount of gelsolin released following Msp treatment and fMLP stimulation. B) Graph represents densitometry analysis mean ± SEM (n = 4, ** P<0.01, unpaired t test).