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. 2013 Jun 3;8(6):e66209. doi: 10.1371/journal.pone.0066209

Figure 8. PTEN inhibition restores Msp-mediated defects in neutrophil polarization and chemotaxis.

Figure 8

Neutrophil migration toward fMLP was assessed using Zigmond chambers. Neutrophils were treated with VO-OHpic and Msp as indicated, attached to coverslips and exposed to an fMLP gradient for 10 minutes. A) The percentage of cells which displayed polarized morphology is shown. B) The total endpoint distance migrated and the velocity of migration is shown. Graphs represent mean ± SEM of 3 independent experiments (** P<0.01, *** P<0.001, unpaired t test). C) Representative plots of cell endpoint location with respect to the origin are shown. Approximately 50 cells were counted per condition.