Figure 6. Inhibition of hPAEC proliferation, increase of FITC-dextran permeability and disruption of intercellular junctions by the SERCA blocker.
(A) The SERCA blocker, BHQ inhibited hPAECs proliferation in a concentration-dependent manner. The bar graph summarizes 3 independent experiments each performed in triplicates. (B) BHQ significantly increased the FITC-dextran permeability of hPAECs. The bar graphs summarize 3 independent experiments each performed in triplicates. (**p<0.01, ***p<0.001 compared to Vehicle control). (C) Confocal microscopic images revealed that BHQ reduced the VE-cadherin signal compared to control, similar to Poly I:C and LY-294002. Nuclei were counterstained with DAPI (blue).