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. 2013 Jun 3;8(6):e61810. doi: 10.1371/journal.pone.0061810

Figure 5. Transactivation of CRT/DRE cis-element containing promoter by SsCBF1 and AtCBF1.

Figure 5

(A) Combinations of reporter and effector constructs used in the transient expression assays. Agrobacterium strain LBA4404 was used as a negative control. The 35Spro:eGFP construct was used as a positive control. The reporter eGFP gene was driven by the wild-type or mutant COR15A promoter. The effectors SsCBF1 and AtCBF1 genes were driven by the CaMV35S promoter. (B) Semi-quantitative PCR detection of SsCBF1, AtCBF1 and ACTIN1 expression in tobacco leaves infiltrated with different combinations of constructs as shown in (A). (C) qRT-PCR analysis of eGFP expression. qRT-PCR procedure was as described in Methods. The results are representative of three independent experiments.