Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 3;8(6):e61810. doi: 10.1371/journal.pone.0061810

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Transient expression assays showing that SsCBF1 and AtCBF1 specifically bind to the CRT elements and activate the expression of LUC reporter gene. The bottom panel indicates the combination of reporter and effector plasmids infiltrated. The LUC reporter gene is driven by a promoter with or without four tandem CRT elements fused upstream of a minimal (−46) 35S promoter sequence (min35S_pro). The effector CBF1 genes are under the control of the CaMV35S promoter. Representative images of N.benthamiana leaves 72 h after infiltration are shown. (B) Quantitative analysis of luminescence intensity in (A). Five independent determinations were assessed. Error bars represent SD. Asterisks denote Student's t-test significance levels compared with the control: ***P<0.001. (C) qRT-PCR analysis of SsCBF1 and AtCBF1 expression in the infiltrated leaf areas shown in (A). Total RNAs were extracted from leaves of N. benthamiana coinfiltrated with the constructs. Five independent determinations were assessed. Error bars represent SD.