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. 2013 Jun 3;8(6):e61810. doi: 10.1371/journal.pone.0061810

Figure 8. SsCBF1 transcript levels in response to various abiotic stresses.

Figure 8

(A) Low-temperature induced expression pattern of SsCBF1 in S. lycopersicoides. S. lycopersicoides seedlings grown under standard conditions were transferred to a climate chamber set at 4°C for a 24 time course under constant light. The aerial parts were harvested for RNA extraction and qRT-PCR analysis. Zero time samples were taken prior to treatment. Transcript levels of SsCBF1 were normalized to the ACTIN2 expression. (B) Drought induced expression pattern of SsCBF1 in S. lycopersicoides. Detached young leaves of S. lycopersicoides were placed on a dry filter paper for drought treatment. Samples were collected according to the time course. The SsCBF1 mRNA levels were analyzed as in (A). (C) High-salinity induced expression pattern of SsCBF1 in S. lycopersicoides. Detached young leaves of S. lycopersicoides were placed on the filter paper soaked with NaCl solution (250 mM, 0.02% Tween-20) for a high-salinity treatment. Samples were collected at the indicated time points. The SsCBF1 mRNA levels were analyzed as in (A). Data shown are average and SD of triplicate reactions. Shown are representative data from one biological replicate and three biological replicates were conducted with similar results.