Figure 9. Freezing tolerance and delayed flowering phenotype of 35S:SsCBF1 transgenic Arabidopsis.
(A) Schematic representation of the construct used for Arabidopsis transformation of the SsCBF1 gene. (B) Relative expression of SsCBF1 in Col-0 and two T3 generation transgenic lines (#11 and #18). Total RNA was extracted from 10-day-old seedlings, then analyzed by semi-quantitative RT-PCR. ACTIN7 gene was used as an internal control. (C) Comparison of freezing tolerance between Col-0 and two transgenic lines. See Methods for details. The right diagram indicates different genotypes used in the assay. (D) Late flowering phenotype of transgenic Arabidopsis overexpressing SsCBF1. Col-0 and two SsCBF1 OE lines were grown under the same conditions as described in Methods. Four-week-old plants were photographed.