Figure 6. Suppression of mitochondrial transcription factor A (Tfam) expression by CsA: reversal by green tea polyphenols.

Rats were fed semi-synthetic powdered diets containing 0% (Control) or 0.1% polyphenol extracts (Polyphenols) starting 3 days prior to CsA treatment (25 mg/kg, i.g. for 3 weeks). Renal Tfam protein (30 kDa) was determined by immunoblotting. Representative images are shown in A and quantification by densitometry is shown in B. Tfam mRNA was determined by qPCR (C). a, p<0.05 vs. control diet+vehicle; b, p<0.05 vs. polyphenol-containing diet+vehicle; c, p<0.05 vs. control diet+CsA (n = 4 per group).