Figure 2. Responsiveness of various Or-Orco complexes to cyclic nucleotides.

(A) Representative current traces of oocytes expressing various insect Or-Orco complex in response to their cognate ligands and cyclic nucleotide reagents. Pheromone/odorant was applied to the oocytes at the time indicated by the arrowhead (for 5 sec), and cyclic nucleotide reagent was applied during the time indicated by colored square (for 30 sec), respectively. Concentrations of ligands are as follows: E11–14:OH, 10 µM, E11–14:OAc, 10 µM, pentylacetate, 100 µM, 2-methylphenol, 100 µM, cyclic nucleotide reagents, 100 µM. (B) Summary of the average responses to cyclic nucleotide reagents. Each response was normalized to a response to the cognate ligand of each Or. The bar graphs show mean ± S.E.M. from 3–4 independent oocytes.