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. Author manuscript; available in PMC: 2013 Jun 3.
Published in final edited form as: Biochemistry. 2004 Sep 28;43(38):12113–12122. doi: 10.1021/bi0494166

Figure 2.

Figure 2

hKeap1 interacts with a region of FAC1 containing a putative PEST domain. (A) GST:FAC1 fusion proteins shown here were used to make affinity columns. GST is shown in black, whereas the remainder contains the indicated FAC1 domains. FAC1 deletion mutants containing the putative PEST domain, nuclear localization sequence (NLS), and a nuclear export sequence (NES) are shown. Relative binding of each FAC1 domain to hKeap1 is indicated on the right. In (B) 35S-labeled, in vitro translated hKeap1 (42–624) (hKeap1; lane 1) was incubated with the following affinity columns: GST (lane 2), GST:FAC1(438–810) (lane 3), GST:FAC1(611–810) (lane 4), GST:FAC1(401–500) (lane 5), and GST:FAC1(501–610). Column-bound proteins were fractionated by size on a 10% SDS–polyacrylamide gel, which was transferred to PVDF. The autoradiograph of the transferred gel is shown in (B).