Skip to main content
. Author manuscript; available in PMC: 2013 Jun 3.
Published in final edited form as: Biochemistry. 2004 Sep 28;43(38):12113–12122. doi: 10.1021/bi0494166

Figure 5.

Figure 5

Coexpression of hKeap1 with FAC1 redistributes FAC1 within the cytoplasm. PT67 murine fibroblasts were transfected with eGFP:hKeap1 alone (A) or with eGFP:hKeap1 and epitope tagged FAC1 (B). Transfected cells were stained for FAC1 by immunohistochemistry, actin by TRITC conjugated phalloidin, and DNA by DAPI and analyzed by quadruple-label immunofluorescent laser confocal microscopy. For panels A and B, FAC1 is shown in red, eGFP:hKeap1 is shown in green, actin is labeled with phalloidin and shown as blue in the Phd panel, and DAPI labeling nuclei are shown as blue in the DAPI panel. Panel B demonstrates FAC1 colocalization with eGFP:hKeap1. In panel C, western analysis of cells transfected with eGFP:hKeap1 (lane 2) and epitope-tagged FAC1 (eFAC1; lane 3) or untransfected (lane 1) demonstrates the levels of eGFP:hKeap1 and eFAC1 upon transfection. Bar = 20 μM.