Fig. 1.

T cell recovery in thymectomized, T cell-depleted mice after syngeneic thymic transplantation. WBCs and spleen cells were prepared from WT thymus-grafted CD47 KO mice (WT → KO; n = 7) and CD47 KO thymus-grafted WT mice (KO → WT; n = 6) at the indicated times and analyzed for T cell recovery by flow cytometry. (A) Percentages(mean ± SEM) of total (□), WT (○) and CD47 KO (●) T cells in the WBCs at weeks 3 and 6 post-thymus transplantation. Because of the low level of CD47 KO T cells, the two lines denoting the percentages of total and WT mouse T cells overlapped in the KO → WT group. (B) Representative flow cytometric profiles of WBCs stained with anti-CD47 plus anti-CD4 (left column) or CD8 (right column). WBCs from untreated WT and CD47 KO mice were used as controls. (C). Percentages (mean ± SEM) of WT and CD47 KO T cells in the spleen at week 12 post-thymus transplantation (n = 4 per group). Representative flow cytometric profiles are shown at the right. Data are combined from two independent experiments.