Fig. 2.

Repopulation of thymic grafts by recipient macrophages. Thymic grafts were harvested from WT thymus-grafted CD47 KO mice (WT → KO; n = 6) and CD47 KO thymus-grafted WT mice (KO → WT; n = 5) 7 weeks after thymic transplantation, and single cell suspensions were prepared and analyzed for SIRPα⁺CD11b⁺ macrophage repopulation. Donor- and recipient-derived cells were distinguished by staining with anti-CD47 mAb, in which recipient cells from WT → KO and KO → WT mice were CD47⁻ and CD47⁺, respectively. Shown are percentages (mean ± SEM) of SIRPα⁺CD11b⁺ macrophages in total thymic cells (A), percentages (mean ± SEM) of host-type SIRPα⁺CD11b⁺ macrophages in total macrophages (B), and representative staining profiles (C).