Skip to main content
. Author manuscript; available in PMC: 2013 Jun 3.
Published in final edited form as: Cell Transplant. 2011 Apr 29;20(0):1915–1920. doi: 10.3727/096368911X566253

Figure 1. Human CD47 expression reduces the susceptibility porcine LCL cells to cytotoxicity by human macrophages.

Figure 1

hCD47-LCL cells and pKS-LCL cells were mixed (at 1:1 ratio) and co-cultured (4×104 /well) with or without human THP-1 macrophages, and the ratios of hCD47-LCL cells to pKS-LCL cells in the cultures were determined every day for 8 days by flow cytometry. A. Flow cytometric analysis of mixed hCD47-LCL cells and pKS-LCL cells stained with anti-human CD47 mAb (prior to co-culture). Open and filled histograms represent hCD47-LCL (hCD47+) and pKS-LCL (hCD47-) cells, respectively. B. Ratios of hCD47-LCL (pMHC-I+hCD47+) to pKS-LCL (pMHC-I+hCD47−) cells in the co-cultures with (○) or without (●) human THP-1 macrophages at the indicated time points. Combined results from 3 independent experiments are presented. *p < 0.01; **p < 0.001.