Table 3.
Primers used to amplify the migration-related genes
| Primer | GenBank accession number | Direction | Sequence (5′ → 3′) |
|---|---|---|---|
| CXCR4 |
NM_003467 |
Sense |
TGGCCGACCTCCTCTTTGT |
| Anti-sense |
AGTTTGCCACGGCATCAACT |
||
| SDF-1 (CXCL12) |
NM_000609 |
Sense |
CCAACGTCAAGCATCTCAAAATT |
| Anti-sense |
AGCCGGGCTCAATCTGAAG |
||
| RANTES (CCL5) |
NM_002985 |
Sense |
TGCCCACATCAAGGAGTATTTCTA |
| Anti-sense |
GCACACACTTGGCGGTTCT |
||
| MCP-1 (CCL2) |
NM_002990 |
Sense |
GCGTGGTGTTGCTAACCTTCA |
| Anti-sense |
GGCTCTTCATTGGCTCAGCTT |
||
| MMP-2 |
NM_004530 |
Sense |
TTTTGATGACGATGAGCTATGGA |
| Anti-sense |
CCATCGGCGTTCCCATACT |
||
| 18S |
NR_003286 |
Sense |
CGGCTACCACATCCAAGGAA |
| Anti-sense | GCTGGAATTACCGCGGCT |