Table 3.
Semi-quantitative immunopositivity (score 0 to 3) and number of positive samples in the diagnostic colonic tissue samples of pediatric ulcerative colitis patients and non inflammatory bowel disease patients
| Surgery (n = 24) | Conservative treatment controls (n = 27) | Non-IBD controls (n = 20) | P value Surgery vs conservative treatment controls | P value Surgery vs non-IBD controls | |
| Number of positive samples n (%) | |||||
| Tryp-1 | |||||
| Epithelium | 13 (57) | 19 (76) | 15 (79) | 0.22 | 0.19 |
| Stroma; inflammatory cells | 11 (48) | 8 (32) | 5 (26) | 0.38 | 0.21 |
| Tryp-2 | |||||
| Epithelium | 21 (95) | 21 (81) | 18 (95) | 0.20 | 0.22 |
| TATI | |||||
| Epithelium | 18 (78) | 24 (92) | 15 (75) | 0.23 | 1.00 |
| MMP-9 | |||||
| Epithelium | 4 (18) | 2 (8) | 0 (0) | 0.40 | 0.17 |
| Stroma; inflammatory cells | 11 (50) | 12 (48) | 1 (5) | 1.00 | 0.0061 |
| Median immunopositivity | |||||
| Tryp-1 | |||||
| Epithelium | 1.0 | 2.0 | 2.0 | 0.032 | 0.042 |
| Stroma; inflammatory cells | 0.0 | 0.0 | 0.0 | 0.16 | 0.10 |
| Tryp-2 | |||||
| Epithelium | 1.0 | 1.0 | 1.0 | 0.56 | 0.38 |
| TATI | |||||
| Epithelium | 1.0 | 1.0 | 1.0 | 0.81 | 0.97 |
| MMP-9 | |||||
| Epithelium | 0.0 | 0.0 | 0.0 | 0.33 | 0.17 |
| Stroma; inflammatory cells | 0.5 | 0.0 | 0.0 | 0.89 | 0.0033 |
1
Both UC patient groups had significantly more positively stained inflammatory cells in their colonic epithelium when compared to the non-IBD controls;
2
The patients who had undergone surgery had significantly lower immunopositivity when compared to the conservatively treated and non-IBD controls;
3
Both UC patient groups had significantly higher immunopositivity when compared to the non-IBD controls. IBD: Inflammatory bowel disease; UC: Ulcerative colitis; TATI: Trypsin inhibitor; Tryp-2: Trypsinogen-2; MMP-9: Matrix metalloproteinase-9.