Fig. 7.

Glucose-induced changes in [Ca²⁺]_c and insulin secretion in MIN6 pseudoislets exposed to PI3K inhibitors. Simultaneous recordings of [Ca²⁺]_c and insulin release in control (a) or LY294002-treated (prior to and during perifusion) pseudoislets (b). Changes in [Ca²⁺]_c in the pseudoislets after acute exposure to LY94002 (c) or wortmannin (d). Whereas the parallel recordings of insulin and [Ca²⁺]_c are representative of three independent experiments, the [Ca²⁺]_c recordings are representative of 26 (a), 13 (b), 7 (c) and 8 (d) pseudoislets, respectively, from between two and eight independent experiments; fluor exc, fluorescence excitation