Table 2.

Expression of mitochondrial respiration and electron transport genes in MIN6 monolayer cells and MIN6 pseudoislets

Gene symbol	Fold regulation (PCR array)	Validation (qRT-PCR)	Gene symbol	Fold regulation (PCR array)	Validation (qRT-PCR)
Lhpp	1.4658		Ndufa7	2.3978	3.10 ± 0.42
Atp4a	−1.1394		Ndufa8	2.0163	1.85 ± 0.13*
Atp5a1	1.8554		Ndufab1	−2.2788	1.92 ± 0.34*
Atp5b	1.7553		Ndufb10	2.073	1.96 ± 0.35*
Atp5c1	1.7432		Ndufb2	1.2939
Atp5d	1.6955		Ndufb3	1.2156
Atp5f1	1.8046		Ndufb4	1.5175
Atp5g1	1.2498		Ndufb5	1.4557
Atp5g2	1.8046		Ndufb6	1.6264
Atp5g3	2.0874	1.61 ± 0.08*	Ndufb7	1.4658
Atp5h	1.3395		Ndufb8	1.6955
Atp5j	1.7798		Ndufb9	1.9208
Atp5j2	1.6378		Ndufc1	2.0303	1.88 ± 0.34*
Atp5o	1.7073		Ndufc2	1.7675
Atp6v0a2	1.5602		Ndufs1	−1.1878
Atp6v1c2	3.4623	2.48 ± 0.59*	Ndufs2	1.1032
Bcs1l	1.7675		Ndufs3	1.7922
Cox11	2.2372	1.73 ± 0.18*	Ndufs4	−1.0485
Cox4i1	2.6239	2.50 ± 0.69*	Ndufs5	1.1742
Cox5a	1.1421		Ndufs6	2.0445	3.58 ± 0.83*
Cox5b	1.6264		Ndufs7	2.2528	2.54 ± 0.47*
Cox6a1	1.5281		Ndufs8	1.9885
Cox6a2	–3.383	−2.37 ± 0.28*	Ndufv1	2.0874	3.17 ± 0.20*
Cox6b1	1.4257		Ndufv2	2.3648	2.42 ± 0.35*
Cox6b2	1.9885		Ndufv3	2.0587	4.41 ± 0.56*
Cox6c	1.8172		Oxa1l	2.2842	2.14 ± 0.38*
Cox7a2	1.1824		Ppa1	−1.8382
Cox7a2l	2.9317	3.17 ± 0.70*	Ppa2	1.285
Cox7b	1.571		Sdha	2.073	1.56 ± 0.17*
Cox8a	2.1019	1.82 ± 0.31*	Sdhb	1.4061
Cox8c	1.6955		Sdhc	1.4159
Cyc1	1.285		Sdhd	1.8298
Ndufa1	1.9476		Uqcr11	1.2326
Ndufa10	1.507		Uqcrc1	1.9612
Ndufa2	1.7073		Uqcrc2	1.9612
Ndufa3	1.604		Uqcrfs1	1.8683
Ndufa4	1.2326		Uqcrh	2.1166	3.5 ± 0.6*
Ndufa5	1.8298		Uqcrq	1.8426
Ndufa6	2.3161	2.77 ± 0.52*

Results are means ± SEM of four separate experiments

Differential mRNA expression levels of genes encoding the components of mitochondrial electron transport and oxidative phosphorylation in MIN6 pseudoislets compared with monolayers was measured by PCR array and validated by quantitative RT-PCR (qRT-PCR)

*p < 0.05 compared with MIN6 monolayer cells