Table 2.
Sequence quality and closest HLA allele designation for heterozygous samples
| Sample | Gene | Average depth | Coverage¶(allele 1/allele 2) | Luminex genotype | Closest HLA genotype |
|---|---|---|---|---|---|
| E1 |
A |
4306.6 |
97.1/100 |
02:01/31:01 |
02:01:01:01/31:01:02 |
| |
C |
6668.5 |
100/99.6 |
03:03/03:04 |
03:03:01/03:04:01:01 |
| |
B |
5250.9 |
100/100 |
15:11/40:01 |
15:11:01*/40:01:02 |
| |
DRB1 |
272.2 |
99.7/95.6 |
04:05/09:01 |
04:05:01/09:01:02* |
| |
DQB1 |
3322.3 |
100/99.5 |
03:03/04:01 |
03:03:02:02/04:01:01 |
| |
DPB1 |
780.8 |
95.5/100 |
02:02/05:01 |
02:02/05:01:01* |
| E8 |
A |
4303.7 |
100† |
24:02/24:02 |
24:02:01:01/24:02:01:01 |
| |
C |
3904.7 |
100/100 |
12:02/14:02 |
12:02:02/14:02:01 |
| |
B |
2829.4 |
99.7/100 |
51:01/52:01 |
51:01:01/52:01:01:01 |
| |
DRB1 |
612.3 |
100/100 |
01:01/15:02 |
01:01:01/15:02:01 |
| |
DQB1 |
3132.6 |
100/100 |
05:01/06:01 |
05:01:01:02/06:01:01 |
| |
DPB1 |
588.7 |
100/100 |
04:02/09:01 |
04:02:01:02/09:01 |
| E11 |
A |
3880.2 |
100/100 |
24:02/31:01 |
24:02:01:01/31:01:02 |
| |
C |
3695.0 |
100/100 |
01:02/07:04 |
01:02:01/07:04:01 |
| |
B |
4490.7 |
100/100 |
15:18/54:01 |
15:18:01*/54:01:01 |
| |
DRB1 |
501.8 |
78.3/99.2 |
04:01/04:05 |
04:01:01/04:05:01 |
| |
DQB1 |
3751.8 |
100/100 |
03:01/04:01 |
03:01:01:01/04:01:01* |
| |
DPB1 |
748.2 |
100/100 |
02:01/05:01 |
02:01:02*/05:01:01* |
| E17 |
A |
808.4 |
100/100 |
02:07/24:02 |
02:07:01/24:02:01:01 |
| |
C |
1708.7 |
100/100 |
01:02/12:02 |
01:02:01/12:02:02 |
| |
B |
2507.7 |
100/100 |
46:01/52:01 |
46:01:01/52:01:01:02 |
| |
DRB1 |
2080.3 |
100/100 |
08:03/15:02 |
08:03:02/15:02:01 |
| |
DQB1 |
1638.1 |
100† |
06:01/06:01 |
06:01:01/06:01:01 |
| |
DPB1 |
1649.6 |
97.9/98.4 |
05:01/09:01 |
05:01:01*/09:01 |
| E25 |
A |
1042.7 |
100/100 |
02:06/24:02 |
02:06:01/24:02:01:01 |
| |
C |
2150.0 |
100/100 |
07:02/12:02 |
07:02:01:03/12:02:02 |
| |
B |
4871.7 |
100/100 |
07:02/52:01 |
07:02:01/52:01:01:02 |
| |
DRB1 |
460.3 |
100/32.8 |
01:01/09:01 |
01:01:01*/09:01:02* |
| |
DQB1 |
1396.3 |
100/100 |
03:03/05:01 |
03:03:02:02/05:01:01:02* |
| |
DPB1 |
2289.0 |
99.6/100 |
02:02/04:02 |
02:02*/04:02:01:01* |
| E28 |
A |
1645.3 |
97.2/100 |
26:01/33:03 |
26:01:01/33:03:01* |
| |
C |
824.8 |
99.7† |
03:04/03:04 |
03:04:01:02/03:04:01:02 |
| |
B |
2799.6 |
100/100 |
40:02/40:06 |
40:02:01*/40:06:01:01 |
| |
DRB1 |
236.5 |
100† |
09:01/09:01 |
09:01:02*/09:01:02* |
| |
DQB1 |
817.5 |
100† |
03:03/03:03 |
03:03:02:02/03:03:02:02 |
| |
DPB1 |
343.0 |
100† |
02:01/02:01 |
02:01:02/02:01:02 |
| E30 |
A |
1711.4 |
100/100 |
24:02/33:03 |
24:02:01:01/33:03:01* |
| |
C |
2670.8 |
100/100 |
01:02/14:03 |
01:02:01*/14:03 |
| |
B |
1899.1 |
100/100 |
44:03/55:02 |
44:03:01/55:02:01 |
| |
DRB1 |
399.4 |
17.1/99.8 |
09:01/13:02 |
09:01:02*/13:02:01* |
| |
DQB1 |
773.1 |
100/97.8 |
03:03/06:04 |
03:03:02:02/06:09* |
| |
DPB1 |
460.4 |
100/100 |
04:01/04:01 |
04:01:01/04:01:01 |
| M13 |
A |
3689.7 |
97.2/100 |
11:01/24:02 |
11:01:01/24:02:01:01 |
| |
C |
3386.3 |
100/100 |
01:02/04:01 |
01:02:01/04:01:01:01 |
| |
B |
3508.5 |
100/100 |
15:01/59:01 |
15:01:01:01/59:01:01:01 |
| |
DRB1 |
1029.3 |
100/99.5 |
04:05/09:01 |
04:05:01/09:01:02* |
| |
DQB1 |
577.6 |
100/100 |
03:03/04:01 |
03:03:02:02/4:01:01 |
| |
DPB1 |
1981.8 |
100/100 |
04:02/05:01 |
04:02:01:02/05:01:01* |
| M14 |
A |
1690.3 |
100/100 |
31:01/33:03 |
31:01:02/33:03:01 |
| |
C |
4353.2 |
100/100 |
04:01/06:02 |
04:01:01:01/06:02:01:01 |
| |
B |
4733.5 |
100/100 |
15:01/57:01 |
15:01:01:01/57:01:01 |
| |
DRB1 |
446.2 |
100/99.5 |
15:01/07:01 |
15:01:01:02*/07:01:01 |
| |
DQB1 |
763.0 |
98.8/98.9 |
03:03/06:02 |
03:03:02:01/06:02:01 |
| |
DPB1 |
1209.5 |
99.8/100 |
09:01/04:02 |
09:01/04:02:01:02* |
| M15 |
A |
1153.2 |
100/100 |
31:01/33:03 |
31:01:02/33:03:01 |
| |
C |
1448.1 |
99.7/99.8 |
14:02/14:03 |
14:02:01/14:03 |
| |
B |
3312.9 |
100/100 |
44:03/51:01 |
44:03:01/51:01:01 |
| |
DRB1 |
463.4 |
100† |
04:05/04:05 |
04:05:01/04:05:01 |
| |
DQB1 |
280.9 |
100† |
04:01/04:01 |
04:01:01*/04:01:01* |
| |
DPB1 |
2932.9 |
99.5/100 |
02:01/03:01 |
02:01:02/03:01:01 |
| M20 |
A |
7631.1 |
100/100 |
02:06/11:01 |
02:06:01/11:01:01 |
| |
C |
7675.7 |
100/100 |
03:03/08:01 |
03:03:01/08:01:01 |
| |
B |
7566.7 |
100/100 |
15:02/35:01 |
15:02:01/35:01:01:01* |
| |
DRB1 |
4746.2 |
100† |
15:01/15:01 |
15:01:01:01/15:01:01:01 |
| |
DQB1 |
6952.9 |
100/100 |
06:01/06:02 |
06:01:01/06:02:01 |
| DPB1 | 4631.3 | 100/99.6 | 05:01/02:02 | 05:01:02/02:02 |
¶ Percentage of the HLA gene covered by reads that passed the Q20 threshold for nucleotide quality.
*Mismatched bases with recorded sequences in IMGT/HLA database observed.
† Homozygous HLA allele.