Table 3.
Specific activities and ranking of lipases in P(3HB- co -92 mol% 4HB), pNPL and olive oil hydrolysis assay
|
Sources of lipases |
P(3HB- co -92 mol% 4HB) |
pNPL |
|
Olive oil |
|
|
|---|---|---|---|---|---|---|
| Specific activity (Relative density/ min/ mg) | Rank* | Specific activity (μmol 4-nitrophenol/ min/ mg) | Rank† | Specific activity (μmol fatty acid/ min/ mg) | Rank# | |
|
C. viscosum |
4369.47 |
1 |
9800 |
1 |
365.00 |
1 |
|
C. antarctica |
596.29 |
2 |
44 |
4 |
0.60 |
11 |
|
M. miehei |
249.67 |
3 |
8.1 |
6 |
49.35 |
2 |
|
P. cepacia |
58.76 |
4 |
80 |
2 |
15.28 |
3 |
|
R. oryzae |
52.8 |
5 |
0.1 |
10 |
6.19 |
6 |
|
R. arrhizus |
45.52 |
6 |
6.2 |
7 |
10.69 |
4 |
| Porcine pancreas |
32.19 |
7 |
0.25 |
8 |
0.90 |
10 |
|
P. fluorescens |
23.97 |
8 |
37 |
5 |
5.64 |
7 |
|
M. javanicus |
21.05 |
9 |
0.15 |
9 |
2.83 |
8 |
|
C. rugosa |
15.21 |
10 |
68 |
3 |
8.72 |
5 |
| R. niveus | 1.06 | 11 | 0.03 | 11 | 1.53 | 9 |
* Rank according to specific activity by using P(3HB-co-4HB) as substrate, †Rank according to specific activity by using p-NPL as substrate, #Rank according to specific activity by using olive oil as substrate.