Table 1.
Antibodies used in the immunohistochemical study
| Antibody | Source | Non-neoplastic mucosa | Altered expression in cancer |
|---|---|---|---|
| Cytokeratin |
mouse monoclonal, clone AE1/AE3, 1:200, Dako |
Cytoplasmic |
Loss |
| E-cadherin |
mouse monoclonal, clone HECD-1, 1:200, Zymed |
Membranous |
Loss |
| TTF-1 |
mouse monoclonal, clone 8G7G3/1, 1:200, Dako |
Nuclear |
Loss |
| β-catenin |
mouse monoclonal, clone CAT-5H10, 1:500, Zymed |
Membranous |
Cytoplasmic or Nuclea |
| Vimentin |
mouse monoclonal, clone vim-3B4, 1:400, Dako |
Negative |
Cytoplasmic |
| Snail |
rabbit polyclonal, ab 85931, 1:500, Abcam |
Negative |
Nuclear |
| Twist |
rabbit polyclonal, ab 50581, 1:500, Abcam |
Negative |
Cytoplasmic |
| CD44 | mouse monoclonal, clone DF1485, 1:250, Dako | Negative | Membranous |