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. 2013 May 7;32(11):1626–1638. doi: 10.1038/emboj.2013.100

Figure 5.

Figure 5

EcR, br-c, srp and pnr are critical for the PGRP-LC-independent hormonal control of AMP gene induction. qRT–PCR analysis of Dpt, Drs and Mtk expression in parental S2* cells (A–C) and PGRP-LCx-FLAG expressing cells (D–F) treated with RNAi targeting for EcR, br-c, Eip78C, Eip93F, Eip74EF, Eip75B, Hr46, pnr, srp or mock transfected. Cells were exposed or not to 20E treatment for 24 h and then stimulated (or not) with PGN for an additional 6 h, as indicated. The results shown represent the mean of three independent experiments and error bars are s.d. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test, *P<0.05, **P<0.01, ***P<0.001.