Figure 1.

The LATS/Mob1 complex is essential for induction of YAP Ser127 and Ser381 phosphorylation by cytoskeletal damage. (A) Phosphorylation at Ser127 and Ser381 by actin cytoskeletal damage. NIH3T3 cells were treated with 5 μM latrunculin B or seeded onto poly-HEMA-coated dishes and incubated for the indicated times. (B) Indispensability of LATS1/2 for YAP phosphorylation. Lats1^−/−;Lats2^fl/fl MEFs were transduced with either empty or Cre retroviruses. Selected cells were treated as indicated. (C) Indispensability of intact LATS/Mob1 complex for YAP phosphorylation. SV40 LT-immortalized Lats1^−/−;Lats2^fl/fl MEFs were complemented with either LATS1 WT, LATS1 R660A or R696A mutants. After Cre infection, cells were treated as indicated. Lanes 13 and 14 were infected with empty virus in place of Cre to measure LATS2 deletion efficiency. (D) Dispensability of MST1/2 for YAP phosphorylation. Mst1^fl/fl;Mst2^+/+vector, Mst1^fl/fl;Mst2^−/− vector, and Mst1^fl/fl;Mst2^−/− Cre MEFs were infected and treated as indicated.