Effects of ablating BAG versus URX neurons on C. elegans lifespan. Survival curves (A, C and E) and mean lifespan and 75th percentile lifespan (B, D and F) of C. elegans following laser ablation of BAG neurons versus ablation controls (n=74–94) (A, B), laser ablation of URX neurons versus ablation controls (n=76–94) (C, D), or simultaneous ablation of BAG and URX neurons versus ablation controls (n=103–120) (E, F). For comparison, single ablation of BAG neurons or URX neurons (n=22–28) was also included in (E) and (F). (A, C and E) show animals pooled from multiple independent experiments; (B, D and F) show analysis from a representative single experiment (n=22–48 per group). *P<0.05, ****P<0.0001, statistics for curve comparisons are shown in the figure. Error bars represent mean±s.e.m.