Figure 7.

Conformational cycle of Ssc1. A schematic diagram focusing on the different conformational states of Ssc1 observed during the conformational cycle of Ssc1. Ssc1 fluctuates between nucleotide-bound and nucleotide-free states. Interactions of Ssc1 with the substrate occur from the nucleotide-bound conformation. When the substrate is bound, no dynamics are observed, neither for the interdomain sensor nor the lid sensor. Upon dissociation of the substrate, Ssc1 returns to the nucleotide-bound conformation and the dynamics resume.